B. pseudomallei K96243

Burkholderia Pseudomallei strain K96243 was a clinical isolate from Thailand. The genome of B. pseudomallei is 7 247 547 bp in size and the G+C content is 68.06%. It has 2 chromosome of 4 074 542 bp and 3 173 005 bp. The genome of B. pseudomallei carries many genomic islands as compared to its related organism, B. mallei, suggesting extensive horizontal transfer.

There are 3 different type III secretion systems (TTSS) are encoded on the chromosomes of this organism. Two of it are similar to plant pathogenic TTSSs, while the third is similar to the Salmonella pathogenicity island, all of which may contribute to pathogenicity. This organism also carries a number of small sequence repeats which may promoter antigenic variation, similar to what was found with the B. mallei genome.

The lineage of the B. pseudomallei is:
Bacteria > Proteobacteria > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Burkholderia > Pseudomallei group > Burkholderia pseudomallei > Burkholderia pseudomallei K96243


reference:
1) http://www.ncbi.nlm.nih.gov/sites/entrez?db=genomeprj&cmd=Retrieve&dopt=Overview&list_uids=178

AA & DNA sequence of P1

AA sequence of P1

MVKTFYITAAPVGAVPKFLDPLEPKFIPHALLELLPADAREATTQALEANGWEAVPAGGI

VREYGYDAPIDLTDYDGAQASASVQDALRNTGWTPCGTVWHRTQTSPSLAQPPLITRTTL

ERLSSVDLVRQIVLQLTTFGWTATEDGSLTWTHERIHSYLSPDFVERMRADKAAVLESLF

DNGWRVCGAGYWQPGKARSPYLPITADGIVDASREALREGAAVVHLHTRATDDQATLAIP

GLNTPIGIGSQRNHIVLDDYDRIVPTMLDLEPSAILNLSTSARGDRRASQSPLRRAHLKR

YGHAQLAPDVASFSPGPVVFQAGGGYDNPNAFLADQLAHFAEVGVRPEIEVFNHTIVENS

VTLYQSPLVKAGVPVLFMLVAAVDQYHRDPVSGDTSDDSLIDVPTRKAIAKLLQAGTDDA

HEKAVELAATQLRPTVDKLRDNFPSCKISLLLPGPFQALLVDVAIALDLDGIRVGLEDAL

NVFDARVPGGVRKACGTGDQVRWLRLELERRGIGIVDAEALRDELGMSRPDVALFRQAEA

ALAHYPADERLVSADTILDALRPIVDTYRKVEDRLATHLASAEALPADPAALAEHVLTAA

RSFGVTIRSFVEELDRYEDHEYLVARYIQVPQALNFARELLVPRGYSIDAYDRALEDYAR

PGKTVTREHASYSVRVDQFKPLPLRCLEYLVGIPCRYNGDYSNVVNLGLRQSPRYSATMA

LLYHALRELTLELRERSNASRKTCGPVWTVLETSANASEPPVRRDIAPDALTAAIDGVDW

VVLPSTPTTNYPLGLKLANGMAQLFHGFVAQIAADPTLRPSRQTHRDTPLRLLAITHSGR

RDDGETVIEASMLHNRFALNADPSGIYFSEESQLIYERLILPRLVDKPAKLAYNERQLVR

RDTAGFPLYQDGSRARRIKAEQIERLPFLKCFAHSSGIATAQQLDVQACRDGERLGLTAD

ELRAFFDRALLVSFGSAADIHLDWLGTSVVDVTAFNDVRSLAGTTSRHYLIQPGEHADVL

QHCLVHTQPADYRYDHATPVWQEGRQGKVVARLTGVFLLDDHARLDDGHSIRRYLAASPL

WLRQWIARFHDAPADAGAHAILRELQASMTDYRSSANQTTRRALA

                 

DNA sequence of P1

ATGGTCAAGACCTTCTACATCACCGCGGCGCCCGTCGGCGCTGTCCCGAAGTTCCTCGAT

CCGCTGGAGCCGAAGTTCATCCCGCACGCATTGCTCGAGCTGCTGCCCGCCGACGCGCGC

GAGGCGACGACCCAGGCCCTCGAGGCCAACGGATGGGAGGCTGTGCCCGCAGGCGGCATC

GTGCGCGAATACGGCTACGACGCGCCGATCGATCTGACCGATTACGACGGCGCGCAAGCG

TCGGCGTCCGTGCAGGATGCGCTGCGCAACACCGGCTGGACACCGTGCGGCACGGTCTGG

CATCGCACGCAGACCTCGCCGTCGCTCGCGCAGCCGCCGCTGATCACGCGCACCACGCTC

GAGCGGCTATCGTCGGTCGATCTGGTCCGCCAGATCGTCCTGCAGCTCACGACGTTCGGC

TGGACCGCCACCGAAGACGGCAGCCTGACCTGGACGCACGAACGAATCCACTCCTATCTG

TCGCCGGACTTCGTCGAACGAATGCGCGCCGACAAAGCGGCCGTGCTCGAATCGCTGTTC

GACAACGGCTGGCGCGTGTGCGGCGCCGGCTACTGGCAGCCGGGCAAGGCGCGCTCGCCC

TATCTGCCGATCACCGCGGACGGCATCGTCGACGCATCGCGCGAGGCGCTGCGCGAAGGC

GCCGCGGTGGTCCACCTGCACACGCGCGCGACCGACGATCAGGCCACGCTCGCGATCCCG

GGGCTGAACACGCCGATCGGCATCGGCTCGCAGCGCAATCACATCGTGCTCGACGATTAC

GACCGAATCGTGCCGACGATGCTCGATCTGGAACCATCCGCCATCCTGAACCTGTCGACG

AGCGCGCGCGGCGATCGCCGCGCGTCGCAAAGCCCGCTGCGGCGTGCGCACCTGAAGCGC

TACGGCCACGCGCAGCTCGCGCCCGACGTCGCATCGTTCAGCCCCGGCCCCGTCGTGTTC

CAGGCGGGCGGCGGCTACGACAATCCGAACGCGTTCCTCGCGGATCAACTCGCTCACTTC

GCGGAAGTCGGCGTACGGCCCGAGATCGAGGTGTTCAACCATACGATCGTCGAGAACTCG

GTCACGCTCTATCAATCGCCGCTCGTGAAGGCCGGCGTTCCGGTGCTGTTCATGCTCGTC

GCCGCGGTCGACCAATACCACCGCGATCCCGTGAGCGGCGACACGAGCGACGATTCGCTG

ATCGACGTGCCCACCCGCAAGGCGATCGCGAAGCTGCTGCAGGCGGGCACCGACGACGCG

CACGAGAAGGCCGTCGAGCTCGCCGCGACGCAGTTGCGCCCGACCGTCGACAAGCTGCGC

GACAACTTCCCGTCGTGCAAGATCTCGCTGCTGCTGCCGGGCCCGTTCCAGGCGCTGCTC

GTCGACGTGGCGATCGCGCTCGATCTCGACGGCATTCGCGTCGGGCTCGAGGACGCGCTG

AACGTCTTCGACGCGCGCGTGCCGGGCGGCGTGCGCAAGGCGTGCGGCACCGGCGATCAG

GTGCGCTGGCTGCGGCTCGAGCTCGAGCGCCGCGGCATCGGCATCGTCGACGCCGAAGCG

CTGCGCGACGAACTCGGCATGTCGCGGCCCGACGTCGCGCTGTTCCGTCAAGCCGAAGCC

GCGCTCGCGCATTATCCGGCCGACGAGCGGCTCGTATCGGCCGACACGATCCTCGACGCG

CTGCGGCCGATCGTCGACACGTACCGCAAGGTCGAGGATCGACTCGCCACCCACCTCGCG

AGCGCCGAAGCGCTGCCGGCGGACCCCGCCGCGCTCGCCGAGCACGTACTGACGGCCGCG

CGCAGCTTCGGCGTGACGATCCGCTCGTTCGTCGAAGAACTCGATCGCTACGAGGACCAC

GAGTATCTGGTCGCGCGCTACATTCAGGTTCCGCAGGCGCTGAACTTCGCGCGCGAGCTG

CTCGTGCCGCGCGGCTATTCGATCGATGCGTACGACCGCGCGCTCGAGGACTACGCGCGC

CCGGGCAAGACCGTGACGCGCGAGCACGCGAGCTACAGCGTGCGCGTCGACCAGTTCAAG

CCGCTGCCGCTGCGCTGCCTCGAATATCTGGTGGGAATTCCTTGCCGCTACAACGGCGAC

TACAGCAATGTCGTCAATCTCGGCTTGCGCCAGAGCCCGCGCTACAGCGCGACGATGGCG

CTGCTCTATCACGCGCTGCGCGAGCTCACGCTCGAGTTGCGCGAGCGCTCGAATGCGTCG

CGCAAGACATGCGGCCCCGTGTGGACCGTGCTCGAGACCTCGGCGAACGCAAGCGAGCCG

CCCGTGCGCCGCGATATCGCGCCCGACGCGCTGACAGCCGCGATCGACGGCGTCGACTGG

GTCGTGCTGCCGAGCACGCCGACCACCAACTACCCGCTCGGCCTCAAGCTGGCGAATGGG

ATGGCGCAGCTGTTCCACGGCTTCGTCGCGCAGATCGCCGCCGATCCGACGCTGCGCCCG

TCGCGGCAGACGCACCGCGACACGCCGCTGCGCCTGCTCGCGATCACGCATTCGGGCCGC

CGCGACGACGGCGAAACGGTGATCGAAGCCAGCATGCTGCACAACCGCTTTGCGCTGAAC

GCGGATCCGTCGGGCATCTACTTCAGCGAGGAGTCGCAACTGATCTACGAGCGGCTGATC

CTGCCGCGCCTCGTCGACAAGCCCGCCAAGCTCGCATACAACGAGCGGCAACTGGTGCGC

CGGGACACGGCCGGCTTCCCGCTGTATCAGGACGGTTCGCGCGCGCGCCGCATCAAGGCC

GAGCAGATCGAGCGGCTGCCGTTTCTCAAGTGCTTCGCGCACAGCTCGGGGATCGCCACC

GCGCAGCAGCTCGACGTGCAGGCATGCCGCGACGGCGAACGGCTCGGCCTCACGGCCGAC

GAACTCCGCGCGTTCTTCGATCGCGCGCTGCTGGTGTCGTTCGGCTCCGCCGCGGACATC

CACCTCGACTGGCTCGGCACCTCGGTCGTCGACGTGACCGCGTTCAACGACGTGCGCAGC

CTCGCCGGCACGACGAGCCGCCATTACTTGATCCAGCCGGGCGAGCATGCGGACGTGCTG

CAGCACTGCCTCGTGCACACGCAGCCCGCCGACTATCGCTACGATCACGCGACGCCGGTC

TGGCAGGAAGGCCGGCAGGGCAAGGTCGTCGCGCGGCTCACGGGCGTGTTCCTGCTCGAC

GATCATGCGCGGCTCGACGACGGCCACTCGATTCGCCGCTACCTCGCCGCGAGCCCGCTG

TGGCTGCGTCAATGGATCGCGCGTTTTCACGACGCGCCCGCCGACGCCGGCGCACACGCG

ATTCTGCGCGAACTGCAGGCGTCGATGACCGACTACCGGTCGAGCGCGAACCAGACGACG

CGGCGAGCACTCGCGTAA

The accession number of BP

Accession number can be written based on two sources; one is from GenBank and the other is from RefSeq.

The accession number is the unique identifier assigned to the entire sequence record when the record is submitted to GenBank. The GenBank accession number is a combination of letters and numbers that are usually in the format of one letter followed by five digits (e.g., M12345) or two letters followed by six digits (e.g., AC123456). The accession number for a particular record will not change even if the author submits a request to change some of the information in the record. GenBank is an annotated collection of all publicly available DNA sequences.
This accession number is the unique identification number for a complete RefSeq sequence record. RefSeq accession numbers are written in the following format: two letters followed by an underscore and six digits (e.g., NT_123456). Refseq are derived from GenBank records but differ in that each RefSeq is a synthesis of information,not an archived unit of primary research data.

The accession number for the chromosome 1 of burkholderia pseudomallei is BX571965 and for the chromosome 2, the accession number is BX571966.

The P1 protein, the accession number is YP_111366. The sequences of P1 protein is found to be on chromosome 2 with the accession number of NC_006351 from DBsource.



REFERENCES:
1. http://www.ornl.gov/sci/techresources/Human_Genome/posters/chromosome/genejargon.shtml
2. http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=handbook&part=ch18
3. http://www.ncbi.nlm.nih.gov/genbank/

Post all info & knowledge on P1 here

Every little scrap of info will be centralise here. The biology of P1 is unknown - we are the 10 blind men with an "elephant" in the room.